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ColoSSoS | Method evaluation and optimisation

Project Number # 2064

ColoSSoS | Method evaluation and optimisation: Investigation of PCR-based methods and feasibility study for whole-genome sequencing

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Water Research Australia has released the ColoSSoS report, Method evaluation and optimisation: Investigation of PCR-based methods and feasibility study for whole-genome sequencing.

As a part of the broader ColoSSoS program, the Jex Lab at the Walter and Eliza Hall Institute of Medical Research (WEHI) was tasked with:

  • Evaluating PCR-based methods to detect SARS-CoV-2 in wastewater;
  • Assessing control and proxy viral material for use in these tests;
  • Testing novel methods to improve viral detection sensitivity;
  • Developing novel methods to verify wastewater detections of SARS-CoV-2; and,
  • Implementing this work within a routine surveillance program for SARS-CoV-2.

In addition WEHI conducted a feasibility study into whole-gene sequencing for variants of SARS-CoV-2 which are of international importance.

Together with ColoSSoS partners, WEHI have been instrumental in setting up a routine testing program and worked with WaterRA and the Victorian Department of Health to implement wastewater testing in Victoria.

 

 

DOWNLOAD REPORT

 

WaterRA members can access this report by registering and logging in, using the button on the top right. Click here to read more about the ColoSSoS national initiative. Click here to access other research reports from ColoSSoS.