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Spread of Antibiotic Resistance in Anaerobic Sludge Digestion

Project Number # 2058

Understanding and Reducing the Spread of Antibiotic Resistance in Anaerobic Sludge Digestion

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Wastewater treatment plants have been recognised as the hotspots of antibiotic resistant bacteria (ARB) and antibiotic resistance genes (ARGs). This project aims to understand and reduce the spread of antibiotic resistance in anaerobic sludge digestion. This project will utilise 16S rRNA sequencing, qPCR and metagenomics to uncover the changes in the occurrence, abundance and diversity of ARB and ARGs in anaerobic sludge digestion under various operating conditions. This will understand the fate of ARGs and ARB in anaerobic digestion, and will identify the optimal operating conditions for reducing the spread of antibiotic resistance in anaerobic digestion. In addition, based on the results of this study, the control and management strategy for antibiotic resistance will also be proposed.